Modric missed Croatia’s surprise 2-1 win over Spain on Tuesday due to a groin problem, but was passed fit to return along with Juventus striker Mario Mandzukic.
Domagoj Vida, Ivan Strinic and Marcelo Brozovic also come back into the Croatian side after being rested against Spain.
Ronaldo spearheads a much-changed Portugal after they were the only team to make the knockout stage having not won any of their three group games.
Raphael Guerreiro, Jose Fonte and Cedric Soares come into the defence, whilst Adrien Silva replaces Joao Moutinho in midfield.
After becoming the first player to score in four separate Euros to rescue Portugal from an early exit in a thrilling 3-3 draw with Hungary on Wednesday, Ronaldo is aiming for another goalscoring record.
The three-time World Player of the Year is one goal away from matching Michel Platini’s record mark of nine in European championship finals.
Croatia (4-2-3-1)
Danijel Subasic; Darijo Srna (capt), Vedran Corluka, Domagoj Vida, Ivan Strinic; Milan Badelj, Luka Modric; Marcelo Brozovic, Ivan Rakitic, Ivan Perisic; Mario Mandzukic
Coach: Ante Cacic (CRO)
Portugal (4-4-2)
Rui Patricio; Cedric Soares, Pepe, Jose Fonte, Raphael Guerreiro; Andre Gomes, William Carvalho, Adrien Silva, Joao Mario; Cristiano Ronaldo (capt), Nani
Coach: Fernando Santos (POR)
Referee: Carlos Velasco Carballo (ESP)
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