Arsenal defeated Olympiakos in Greece 3-0 thanks to a hat-trick from French striker Olivier Giroud to secure second place in Group F behind Bayern Munich.
Jose Mourinho’s Chelsea saw off his former team Porto 2-0 at Stamford Bridge to take top spot in Group G.
Dynamo Kiev leapfrogged Porto into the second qualification place thanks to a 1-0 win over Maccabi Tel Aviv.
Barcelona, who had already qualified for the next round as Group E winners, drew 1-1 at Bayer Leverkusen which allowed Roma to take second spot after a 0-0 draw at BATE Borisov.
Valencia, with Gary Neville in the coaching hot-seat for the first time, were unable to make it four Spanish teams going through when they lost 2-0 at home to Lyon in Group H.
Zenit St Petersburg had already topped Group H and they were joined in the next stage by tournament debutants Gent, who beat the Russians 2-1.
The draw for the last 16 takes place at UEFA’s headquarters in Nyon next Monday.
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